JRC GMO-Amplicons | EURL GMFF

Record ID:	LQ717905.1
Database:	emblrelpat	Go to original record
Description:	Sequence 11 from Patent EP3323881.
Taxon:	synthetic construct
Original size:	8185 bp
Patents:	EP3323881 (lens, espacenet)

View other 1 match for this record

Method:	QL-ELE-00-002	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of Neomycin phosphotransferase II gene (ISO/FDIS 21569:2005).
Target:	nptII

View other 59426 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
1	1	6786	6999	+	214 bp

Amplicon:

214 bp

1     ctcgccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg
51    cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa
101   acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
151   aggatgatct ggacaagagc atcaggggct cgcgccagcc gaactgttcg
201   ccaggctcaa ggcc

View other 8 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	95.2	21 bp	1	0	1	21	1.42e-07
primerB	100.0	20 bp	0	0	213	194	1.09e-08

Match details