Match details

Record ID:	LP765497.1
Database:	emblrelpat	Go to original record
Description:	Sequence 2 from Patent EP3277709.
Taxon:	synthetic construct
Original size:	14262 bp
Patents:	EP3277709 (lens, espacenet)

View other 22 matches for this record

Method:	QL-ELE-00-015	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of Figwort Mosaic Virus 35S promoter
Target:	P-FMV

View other 631 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
2	0	346	423	+	78 bp

Amplicon:

78 bp

1     caaaataacg tggaaaagag ctgtcctgac agcccactca ctattgcgtt
51    tgacgaacgc agtgacgacc acaaaact

View other 22 matches with an identical amplicon

Primers and probe alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	22 bp	0	0	1	22	3.36e-10
primerB	100.0	18 bp	0	0	76	59	4.97e-08
probe	95.5	22 bp	1	0	26	47	1.56e-08

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101.

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.