Match details

Record ID:	LP737681.1
Database:	emblrelpat	Go to original record
Description:	Sequence 18 from Patent EP3302030.
Taxon:	synthetic construct
Original size:	122 bp
Patents:	EP3302030 (lens, espacenet)

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Method:	QT-ELE-00-001	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Quantitative PCR method for detection of Cauliflower Mosaic Virus 35S promoter (Feinberg et al., 2005).
Target:	CaMV P-35S

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Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	2	45	122	-	78 bp

Amplicon:

78 bp

1     gtcttcaaag caagtggatt gatgtgatat ctccactgac gtaagggatg
51    acgcacaatc ccactatcct tcgcaaga

View other 1 match with an identical amplicon

Primers and probe alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	21 bp	0	0	1	21	1.21e-09
primerB	100.0	22 bp	0	0	78	57	3.36e-10
probe	100.0	26 bp	0	0	30	55	2.48e-12

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101.

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.