JRC GMO-Amplicons | EURL GMFF

Record ID:	LG025702.1
Database:	emblrelpat	Go to original record
Description:	KR 1020150006469-A/185: METHODS AND COMPOSITIONS FOR NUCLEASE-MEDIATED TARGETED INTEGRATION OF TRANSGENES.
Taxon:	synthetic construct
Original size:	6661 bp
Patents:	KR1020150006469 (lens, espacenet)

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Method:	QL-CON-00-005	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of the junction between the pat gene and the CaMV35S terminator (ISO/FDIS 21569:2005).
Target:	pat-CaMV T-35S

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Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	205	412	-	208 bp

Amplicon:

208 bp

1     atggtggatg gcatgatgtt ggtttttggc aaagggattt tgagttgcca
51    gctcctccaa ggccagttag gccagttacc cagatctaat agagtccgca
101   aaaatcacca gtctctctct acaaatctat ctctctctat ttttctccag
151   aataatgtgt gagtagttcc cagataaggg aattagggtt cttatagggt
201   ttcgctca

View other 35 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	21 bp	0	0	1	21	2.95e-09
primerB	100.0	23 bp	0	0	208	186	2.59e-10

Match details