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Food, Farming, Fisheries
European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: LF948615.1
Database: emblrelpat Go to original record
Description: JP 2016198097-A/1: AAD-12 EVENT 416, RELATED TRANSGENIC SOYBEAN LINES, AND EVENT-SPECIFIC IDENTIFICATION THEREOF.
Taxon: synthetic construct
Original size: 10212 bp
Patents: JP2016198097 (lens, espacenet)

View other 3 matches for this record

Method: QT-EVE-GM-013 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Quantitative PCR method for detection of soybean event DAS-68416-4 (Savini et al., 2014).
Target:DAS-68416-4

View other 18 matches for this method

Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 9073 9202 + 130 bp
Amplicon: 130 bp 
1     gtacattaaa aacgtccgca atgtgttatt aagttgtcta agcgtcaata
51    ttttaattct taacaatcaa tattttaatt cttaaacttt attaaatcta
101   acaataaact gtaagaacta attcttaaac

View other 18 matches with an identical amplicon

Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 26 bp 0 0 1 26 4.02e-12
primerB 100.0 33 bp 0 0 130 98 6.97e-16
primerB 100.0 12 bp 0 0 87 76 3.29e-04
probe 100.0 22 bp 0 0 29 50 5.76e-10

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.