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European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: LF151002.1
Database: emblrelpat Go to original record
Description: JP 2016512048-A/10: ENGINEERING PLANT GENOMES USING CRISPR/Cas SYSTEMS.
Taxon: synthetic construct
Original size: 4940 bp
Patents: JP2016512048 (lens, espacenet)

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Method: QT-ELE-00-001 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Quantitative PCR method for detection of Cauliflower Mosaic Virus 35S promoter (Feinberg et al., 2005).
Target:CaMV P-35S

View other 10904 matches for this method

Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 2 4803 4882 + 80 bp
Amplicon: 80 bp 
1     gtcttcaaaa gcaagtggat tgatgtgata tctccactga cgtaagggga
51    tgacgcacaa tcccactatc ctttcgcaag
Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 95.5 22 bp 0 1 1 22 5.78e-08
primerB 95.5 22 bp 0 1 80 59 5.78e-08
probe 96.3 27 bp 0 1 31 57 1.19e-10

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.