JRC GMO-Amplicons | EURL GMFF

Record ID:	LF151002.1
Database:	emblrelpat	Go to original record
Description:	JP 2016512048-A/10: ENGINEERING PLANT GENOMES USING CRISPR/Cas SYSTEMS.
Taxon:	synthetic construct
Original size:	4940 bp
Patents:	JP2016512048 (lens, espacenet)

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Method:	QL-ELE-00-001	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of Cauliflower Mosaic Virus 35S promoter (BVL L 00.00-31, 1998).
Target:	CaMV P-35S

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Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	4676	4872	+	197 bp

Amplicon:

197 bp

1     gctcctacaa atgccatcat tgcgataaag gaaaggccat cgttgaagat
51    gcctctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat
101   cgtggaaaaa gaagacgttc caaccacgtc ttcaaaagca agtggattga
151   tgtgatatct ccactgacgt aaggggatga cgcacaatcc cactatc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	19 bp	0	0	1	19	3.13e-08
primerB	100.0	20 bp	0	0	197	178	9.38e-09

Match details