European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record GM979720.1 details
Record ID: GM979720.1
Database: emblrelpat Go to original record
Description: Sequence 124 from Patent WO2008147935.
Taxon: synthetic construct
Original size: 11615 bp
Patents: WO2008147935 (lens, espacenet)

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Method QL-ELE-00-017 details
Method: QL-ELE-00-017 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description: Qualitative PCR method for detection of Cauliflower Mosaic Virus 35S promoter (Barbau-Piednoir et al., 2014).
Target: CaMV P-35S

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Predicted amplification details:

Predicted amplification details
Mismatches Gaps From To Strand Amplicon size
1 0 2672 2746 - 75 bp

Amplicon size
Amplicon: 75 bp
1     AAAGCAAGTG GATTGATGTG ACATCTCCAC TGACGTAAGG GATGACGCAC
51    AATCCCACTA TCCTTCGCAA GACCC 

View other 969 matches with an identical amplicon


Primers alignment:

Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 21 bp 0 0 2746 2726 3.64e-08
primerB 100.0 20 bp 0 0 2672 2691 1.15e-07


Related publications:

  • Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101.
  • Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.