Match details

Record ID: JB078563.1
Database: emblrelpat Go to original record
Description: Sequence 5 from Patent EP2430160.
Taxon: synthetic construct
Original size: 12259 bp
Patents: EP2430160 (lens, espacenet)

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Method: QL-ELE-00-023 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of T35S pCAMBIA sequences (Rischitor et al., 2016).
Target:T35S pCAMBIA

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
2 0 6765 6901 + 137 bp

Amplicon: 137 bp
1     ccggggatct ggattttagt actggatttt ggttttagga attagaaatt
51    ttattgatag aagtatttta caaatacaaa tacatactaa gggtttctta
101   tatgctcaac acatgagcga aaccctataa gaaccct 

View other 9 matches with an identical amplicon


Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 19 bp 0 0 3 21 2.66e-08
primerB 95.7 23 bp 1 0 137 115 7.81e-09


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.