Match details
| Record ID: | A32909.1 | |
|---|---|---|
| Database: | emblrelpat | Go to original record |
| Description: | Chimeric endochitinase gene | |
| Taxon: | Nicotiana tabacum | |
| Original size: | 1863 bp | |
| Patents: | WO9201792 (lens, espacenet) | |
View other 12 matches for this record
| Method: | QL-ELE-00-011 | Go to GMOMETHODS record |
|---|---|---|
| View in JRC GMO-Matrix | ||
| Description: | Qualitative PCR method for detection of nopaline synthase terminator. | |
| Target: | T-nos | |
View other 14196 matches for this method
Predicted amplification details:
| Mismatches | Gaps | From | To | Strand | Amplicon size |
|---|---|---|---|---|---|
| 0 | 0 | 1715 | 1798 | + | 84 bp |
| Amplicon: | 84 bp |
|---|
1 CATGTAATGC ATGACGTTAT TTATGAGATG GGTTTTTATG ATTAGAGTCC 51 CGCAATTATA CATTTAATAC GCGATAGAAA ACAA
View other 28057 matches with an identical amplicon
Primers and probe alignment:
| Primer/Probe | % Identity | Length | Mismatches | Gaps | From | To | Score |
|---|---|---|---|---|---|---|---|
| primerA | 100.0 | 25 bp | 0 | 0 | 1715 | 1739 | 2.86e-11 |
| primerB | 100.0 | 25 bp | 0 | 0 | 1798 | 1774 | 2.86e-11 |
| probe | 100.0 | 28 bp | 0 | 0 | 1742 | 1769 | 5.41e-13 |
Related publications:
- Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101.
- Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.