Match details

Record ID: HZ183141.1
Database: emblrelpat Go to original record
Description: JP 2015516162-A/284: METHODS AND COMPOSITIONS FOR NUCLEASE-MEDIATED TARGETED INTEGRATION OF TRANSGENES.
Taxon: synthetic construct
Original size: 6661 bp
Patents: JP2015516162 (lens, espacenet)

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Method: QT-CON-00-005 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Quantitative PCR method for detection of the junction between the phosphinothricin N-acetyltransferase gene and the CaMV 35S terminator.
Target:pat-CaMV T-35S

View other 222 matches for this method


Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 205 351 - 147 bp

Amplicon: 147 bp
1     gccagttagg ccagttaccc agatctaata gagtccgcaa aaatcaccag
51    tctctctcta caaatctatc tctctctatt tttctccaga ataatgtgtg
101   agtagttccc agataaggga attagggttc ttatagggtt tcgctca 

View other 35 matches with an identical amplicon


Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 21 bp 0 0 1 21 2.06e-09
primerB 100.0 24 bp 0 0 147 124 5.32e-11


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.