Match details
Record ID: |
MA886850.1 |
Database: |
emblrelpat |
Go to original record |
Description: |
WO 2019054445-A/3: Transgenic Plant, Producing Method for Transgenic Plant, Polynucleotide, Cluster of Polynucleotide, Vector, and Kit. |
Taxon: |
Arabidopsis thaliana |
Original size: |
8980 bp |
Patents: |
WO2019054445 (lens, espacenet)
|
View other 4 matches for this record
Method: |
QL-ELE-00-013 |
Go to GMOMETHODS record |
|
View in JRC GMO-Matrix
|
Description: | Qualitative duplex PCR method for detection of Cauliflower Mosaic Virus 35S promoter and nopaline synthase terminator (partim T-nos) (Waiblinger et al., 2008). |
Target: | T-nos |
View other 15584 matches for this method
Predicted amplification details: |
Mismatches |
Gaps |
From |
To |
Strand |
Amplicon size |
2 |
0 |
5270 |
5353 |
+ |
84 bp |
|
1 catgtaatgc atgacgttat ttatgagatg ggtttttatg attagagtcc
51 cgcaattata catttaatac gcgatagaaa actg
View other 3 matches with an identical amplicon
Primers and probe alignment: |
Primer/Probe |
% Identity |
Length |
Mismatches |
Gaps |
From |
To |
Score |
primerA |
100.0 |
25 bp |
0 |
0 |
1 |
25 |
9.20e-12 |
primerB |
100.0 |
23 bp |
0 |
0 |
82 |
60 |
1.19e-10 |
probe |
100.0 |
28 bp |
0 |
0 |
28 |
55 |
2.28e-13 |
|
Related publications:
Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101.
Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.