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Food, Farming, Fisheries
European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: DD139994.1
Database: emblrelpat Go to original record
Description: BIOLUMINESCENT PLANTS AND METHODS OF MAKING SAME.
Taxon: synthetic construct
Original size: 11478 bp
Patents: JP2005503122 (lens, espacenet)

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Method: QL-ELE-00-005 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of Cauliflower Mosaic Virus 35S promoter (ISO/FDIS 21569:2005).
Target:CaMV P-35S

View other 10854 matches for this method

Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
1 2 10402 10599 - 198 bp
Amplicon: 198 bp 
1     gctcctacaa atgccatcat tgcgataaag gaaaggccat cgttgaagat
51    gcctctgccg acagtggtcc caaaagatgg anccccaccc acgaggagca
101   tcgtggaaaa aagaagacgt tccaacccac gtcttcaaag caagtggatt
151   gatgtgatat ctnccctggn cgtanggatg acgcacaatc ccctnttc

View other 3 matches with an identical amplicon

Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 19 bp 0 0 1 19 3.15e-08
primerB 100.0 14 bp 0 0 192 179 2.04e-05

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.