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Food, Farming, Fisheries
European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: CS559097.1
Database: emblrelpat Go to original record
Description: Sequence 12 from Patent WO2006032426.
Taxon: synthetic construct
Original size: 10765 bp
Patents: WO2006032426 (lens, espacenet)

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Method: QL-ELE-00-025 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative duplex PCR method for detection of pat gene; bar gene (partim pat) (Debode et al., 2016).
Target:pat

View other 1741 matches for this method

Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
2 0 4500 4607 - 108 bp
Amplicon: 108 bp 
1     cgcggtttgt gacatcgtta accattacat tgagacgtct acagtgaact
51    ttaggacaga gccacaaaca ccacaagagt ggattgatga cctagagagg
101   ttgcaaga

View other 56 matches with an identical amplicon

Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 95.5 22 bp 1 0 1 22 2.21e-08
primerB 96.0 25 bp 1 0 108 84 5.58e-10
probe 100.0 29 bp 0 0 53 81 8.17e-14

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.