Match details

Record ID: HV199243.1
Database: emblrelpat Go to original record
Description: JP 2001505412-A/7: Encapsulation of polypeptides within the starch matrix.
Taxon: Zea mays
Original size: 2097 bp
Patents: JP2001505412 (lens, espacenet)

Method: QT-TAX-ZM-006 Go to GMOMETHODS record
Description:Quantitative PCR method for detection of maize starch synthase IIb gene
Target:maize starch synthase IIb

View other 41 matches for this method


Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 293 443 + 151 bp

Amplicon: 151 bp
1     ctcccaatcc tttgacatct gctccgaagc aaagtcagag cgctgcaatg
51    caaaacggaa cgagtggggg cagcagcgcg agcaccgccg cgccggtgtc
101   cggacccaaa gctgatcatc catcagctcc tgtcaccaag agagaaatcg
151   a 

View other 38 matches with an identical amplicon


Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 22 bp 0 0 1 22 6.29e-10
primerB 100.0 23 bp 0 0 151 129 1.86e-10
probe 100.0 25 bp 0 0 28 52 1.60e-11


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.