Match details

Record ID:	MP495297.1
Database:	emblrelpat	Go to original record
Description:	Sequence 49 from Patent WO2020025963.
Taxon:	Tobacco rattle virus
Original size:	20173 bp
Patents:	WO2020025963 (lens, espacenet)

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Method:	QT-ELE-00-004	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Quantitative PCR method for detection of Cauliflower Mosaic Virus 35S promoter
Target:	CaMV P-35S

View other 15625 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
1	0	196	277	+	82 bp

Amplicon:

82 bp

1     gcctctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat
51    cgtggaaaaa gaagacgtcc caaccacgtc tt

View other 17 matches with an identical amplicon

Primers and probe alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	18 bp	0	0	1	18	4.54e-08
primerB	95.5	22 bp	1	0	82	61	1.65e-08
probe	100.0	22 bp	0	0	21	42	3.55e-10

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101.

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.