Match details

Record ID: MV639003.1
Database: emblrelpat Go to original record
Description: Sequence 285 from patent US 10760089.
Taxon: unidentified
Original size: 811 bp
Patents: US10760089 (lens, espacenet)

Method: QT-EVE-BN-012 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Quantitative PCR method for detection of oilseed rape event LBFLFK locus1 (EURL GMFF, 2022).
Target:BPS-BFLFK-2 locus1

View other 39 matches for this method


Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 175 303 + 129 bp

Amplicon: 129 bp
1     accatcatac tcattgctga tccatgtaga tttcccggac atgaagccat
51    ttactctgac cctactccac aaatatattt ttatttataa aaaggtggcc
101   attgtatact atgtgtgcgt atacaggaa 

View other 28 matches with an identical amplicon


Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 23 bp 0 0 1 23 1.58e-10
primerB 100.0 25 bp 0 0 129 105 1.36e-11
probe 100.0 26 bp 0 0 24 49 3.98e-12


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.