Match details

Record ID: CS159296.1
Database: emblrelpat Go to original record
Description: Sequence 3 from Patent WO2005080579.
Taxon: synthetic construct
Original size: 15126 bp
Patents: WO2005080579 (lens, espacenet)

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Method: QL-ELE-00-017 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of Cauliflower Mosaic Virus 35S promoter (Barbau-Piednoir et al., 2014).
Target:CaMV P-35S

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 7189 7262 + 74 bp

Amplicon: 74 bp
1     aaagcaagtg gattgatgtg atatctcact gacgtaaggg atgacgcaca
51    atcccactat ccttcgcaag accc 

View other 3 matches with an identical amplicon


Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 23 bp 0 0 1 23 9.36e-11
primerB 100.0 20 bp 0 0 74 55 3.63e-09


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.