Match details
| Record ID: | MV813346.1 | |
|---|---|---|
| Database: | emblrelpat | Go to original record |
| Description: | Sequence 5 from patent US 10808258. | |
| Taxon: | unidentified | |
| Original size: | 8688 bp | |
| Patents: | US10808258 (lens, espacenet) | |
View other 19 matches for this record
| Method: | QL-ELE-00-018 | Go to GMOMETHODS record |
|---|---|---|
| View in JRC GMO-Matrix | ||
| Description: | Qualitative PCR method for detection of nopaline synthase terminator (Barbau-Piednoir et al., 2014). | |
| Target: | T-nos | |
View other 14185 matches for this method
Predicted amplification details:
| Mismatches | Gaps | From | To | Strand | Amplicon size |
|---|---|---|---|---|---|
| 1 | 0 | 8322 | 8390 | + | 69 bp |
| Amplicon: | 69 bp |
|---|
1 GATTAGAGTC CCGCAATTAT ACATTTAATA CGCGATAGAA AACAAAATAT 51 AGCGCGCAAA CTAGGATAA
View other 14038 matches with an identical amplicon
Primers alignment:
| Primer/Probe | % Identity | Length | Mismatches | Gaps | From | To | Score |
|---|---|---|---|---|---|---|---|
| primerA | 100.0 | 28 bp | 0 | 0 | 3457 | 3484 | 2.41e-12 |
| primerA | 100.0 | 28 bp | 0 | 0 | 8322 | 8349 | 2.41e-12 |
| primerB | 96.0 | 25 bp | 1 | 0 | 3525 | 3501 | 8.43e-10 |
| primerB | 96.0 | 25 bp | 1 | 0 | 8390 | 8366 | 8.43e-10 |
Related publications:
- Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101.
- Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.