Match details

Record ID: CS047646.1
Database: emblrelpat Go to original record
Description: Sequence 4 from Patent WO2005019459.
Taxon: synthetic construct
Original size: 11533 bp
Patents: WO2005019459 (lens, espacenet)

View other 11 matches for this record


Method: QL-ELE-00-021 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of phosphinothricin N-acetyltransferase (pat) gene (Barbau-Piednoir et al., 2014).
Target:pat

View other 1706 matches for this method


Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
2 0 5268 5376 - 109 bp

Amplicon: 109 bp
1     ccgcggtttg tgacatcgtt aaccattaca ttgagacgtc tacagtgaac
51    tttaggacag agccacaaac accacaagag tggattgatg acctagagag
101   gttgcaaga 

View other 21 matches with an identical amplicon


Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 95.0 20 bp 1 0 1 20 2.54e-07
primerB 96.0 25 bp 1 0 109 85 5.64e-10


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.