Match details
| Record ID: |
MM114631.1 |
| Database: |
emblrelpat |
Go to original record |
| Description: |
Sequence 7 from patent US 10106811. |
| Taxon: |
unidentified |
| Original size: |
3316 bp |
| Patents: |
US10106811 (lens, espacenet)
|
View other 7 matches for this record
| Method: |
QL-CON-00-013 |
Go to GMOMETHODS record |
|
View in JRC GMO-Matrix
|
|
| Description: | Qualitative PCR method for detection of the junction between the maize ubiquitin promoter and the modified cry1Ab/1Ac genes (Grohmann et al.,2015). |
| Target: | P-ubi-cry1Ab/Ac |
View other 1 match for this method
| Predicted amplification details: |
| Mismatches |
Gaps |
From |
To |
Strand |
Amplicon size |
| 2 |
2 |
1932 |
2026 |
+ |
95 bp |
|
1 atttgcttgg tactgtttct tttgtcgatg ctcaccctgt tgtttggtgt
51 tacttctgca gtgcaggtcg actctagagg atccatgatg cacaa
| Primers and probe alignment: |
| Primer/Probe |
% Identity |
Length |
Mismatches |
Gaps |
From |
To |
Score |
| primerA |
100.0 |
26 bp |
0 |
0 |
1 |
26 |
3.06e-12 |
| primerB |
100.0 |
17 bp |
0 |
0 |
87 |
71 |
2.94e-07 |
| probe |
100.0 |
27 bp |
0 |
0 |
34 |
60 |
8.92e-13 |
|
Related publications:
Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101.
Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.
