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Food, Farming, Fisheries
European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: KH705541.1
Database: emblrelpat Go to original record
Description: Sequence 21 from patent US 9605273.
Taxon: unidentified
Original size: 10471 bp
Patents: US9605273 (lens, espacenet)

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Method: QT-EVE-GH-011 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Quantitative PCR method for detection of cotton event DAS-81910-7 (EURL GMFF, 2019).
Target:DAS-81910-7

View other 6 matches for this method

Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 1302 1421 + 120 bp
Amplicon: 120 bp 
1     aagcttaggt gatttcgatg atgatatatg tgtatataca taagtatatt
51    tccagtcagc atcatcacac caaaagttag gcccgaatag tttgaaatta
101   gaaagctcgc aattgaggtc

View other 6 matches with an identical amplicon

Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 23 bp 0 0 1 23 1.56e-10
primerB 100.0 20 bp 0 0 120 101 6.05e-09
probe 100.0 20 bp 0 0 66 85 6.05e-09

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.