Match details

Record ID: KH569598.1
Database: emblrelpat Go to original record
Description: Sequence 30 from patent US 9574208.
Taxon: unidentified
Original size: 52256 bp
Patents: US9574208 (lens, espacenet)

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Method: QL-ELE-00-022 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of phosphinothricin N-acetyltransferase (bar) gene (Barbau-Piednoir et al., 2014).
Target:bar

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
1 0 46682 46750 + 69 bp

Amplicon: 69 bp
1     cgtcaaccac tacatcgaga caagcacggt caacttccgt accgagccgc
51    aggaaccgca ggactggac 

View other 42 matches with an identical amplicon


Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 23 bp 0 0 1 23 8.68e-11
primerB 94.7 19 bp 1 0 69 51 5.26e-07


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.