Match details

Record ID: KH534075.1
Database: emblrelpat Go to original record
Description: Sequence 2 from patent US 9562267.
Taxon: unidentified
Original size: 1278 bp
Patents: US9562267 (lens, espacenet)

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Method: QT-TAX-GM-009 Go to GMOMETHODS record
Description:Quantitative PCR method for detection of soybean lectin gene (Mazzara et al., 2007).
Target:soybean lectin

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
2 0 719 823 + 105 bp

Amplicon: 105 bp
1     cttctttctc gcaccaattg acactaagcc acaaacacat gcaggttatc
51    ttggtctttt caacgaaaac gagtctggtg atcaagtcgt cgctgttgag
101   tttga 

View other 11 matches with an identical amplicon


Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 20 bp 0 0 4 23 6.31e-09
primerB 100.0 20 bp 0 0 105 86 5.26e-09
probe 100.0 26 bp 0 0 29 54 3.40e-12


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.