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Food, Farming, Fisheries
European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: KH534075.1
Database: emblrelpat Go to original record
Description: Sequence 2 from patent US 9562267.
Taxon: unidentified
Original size: 1278 bp
Patents: US9562267 (lens, espacenet)

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Method: QT-TAX-GM-004 Go to GMOMETHODS record
Description:Quantitative PCR method for detection of soybean lectin gene (Hird et al., 2003).
Target:soybean lectin

View other 19 matches for this method

Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 590 659 + 70 bp
Amplicon: 70 bp 
1     tggtcgcgcc ctctactcca cccccatcca catttgggac aaagaaaccg
51    gtagcgttgc cagcttcgcc

View other 14 matches with an identical amplicon

Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 18 bp 0 0 1 18 4.19e-08
primerB 100.0 16 bp 0 0 70 55 4.65e-07
probe 100.0 29 bp 0 0 54 26 5.43e-14

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.