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Food, Farming, Fisheries
European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: KH407148.1
Database: emblrelpat Go to original record
Description: Sequence 45 from patent US 9512187.
Taxon: unidentified
Original size: 1857 bp
Patents: US9512187 (lens, espacenet)

View other 1 match for this record

Method: QL-ELE-00-016 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of cry1Ab/Ac gene
Target:cry1Ab/Ac

View other 196 matches for this method

Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
1 0 382 455 + 74 bp
Amplicon: 74 bp 
1     gtggaaatgc gtattcaatt caacgacatg aacagcgcct tgaccacagc
51    tatcccattg ttcgcagtcc agaa

View other 21 matches with an identical amplicon

Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 22 bp 0 0 3 24 3.55e-10
primerB 100.0 20 bp 0 0 74 55 3.63e-09
probe 100.0 25 bp 0 0 26 50 8.04e-12

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.