Match details

Record ID: HK369154.1
Database: emblrelpat Go to original record
Description: Sequence 13 from patent US 8936937.
Taxon: unidentified
Original size: 10606 bp
Patents: US8936937 (lens, espacenet)

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Method: QL-ELE-00-004 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of Cauliflower Mosaic Virus 35S promoter (Lipp et al.,2001).
Target:CaMV P-35S

View other 10536 matches for this method


Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
2 2 10486 10606 + 121 bp

Amplicon: 121 bp
1     ccacgtcttc aaagcaagtg gattgatgtg atatctccac tgacgtaagg
51    gatgacgcac aatcccacta tccttcgcaa gaccttcctc tatataagga
101   agttcatttc atttggagag g 

View other 29 matches with an identical amplicon


Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 21 bp 0 0 1 21 1.81e-09
primerB 100.0 24 bp 0 0 121 98 4.58e-11


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.