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European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: BD295561.1
Database: emblrelpat Go to original record
Description: WO 2003027283-A/2: Nucleic acid fragment for competitive PCR, and kit and method for quantitative determination of recombinant gene.
Taxon: synthetic construct
Original size: 402 bp
Patents: WO2003027283 (lens, espacenet)

View other 6 matches for this record

Method: QT-CON-00-001 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Quantitative PCR method for detection of the junction between the chloroplast transit peptide and the CP4 epsps gene.
Target:CTP-CP4 epsps

View other 26 matches for this method

Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 261 402 + 142 bp
Amplicon: 142 bp 
1     cctttaggat ttcagcatca gtggctacag cgacgttcag aattccgtgt
51    aactgcatgc ttcacggtgc aagcagccgg cccgcaaccg cccgcaaatc
101   ctctggcctt tccggaaccg tccgcattcc cggcgacaag tc

View other 1 match with an identical amplicon

Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 24 bp 0 0 1 24 5.13e-11
primerB 100.0 18 bp 0 0 142 125 8.08e-08
probe 100.0 19 bp 0 0 83 101 2.42e-08

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.