Match details

Record ID: BD295560.1
Database: emblrelpat Go to original record
Description: WO 2003027283-A/1: Nucleic acid fragment for competitive PCR, and kit and method for quantitative determination of recombinant gene.
Taxon: synthetic construct
Original size: 346 bp
Patents: WO2003027283 (lens, espacenet)

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Method: QL-TAX-GM-008 Go to GMOMETHODS record
Description:Qualitative PCR method for detection of soybean lectin gene (EU-Project SMT4-CT96-2072:1998).
Target:soybean lectin

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 1 139 + 139 bp

Amplicon: 139 bp
1     gccctctact ccacccccat ccacatttgg gacaaagaaa ccggtagcgt
51    tgccagcttc ttagcactga cgtctgaatg tgccgcttcc ttcaacttca
101   ccttctatgc ccctgacaca aaaaggcttg cagatgggc 

View other 11 matches with an identical amplicon


Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 22 bp 0 0 1 22 5.77e-10
primerB 100.0 23 bp 0 0 139 117 1.70e-10


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.