Match details
Record ID: |
BD249874.1 |
Database: |
emblrelpat |
Go to original record |
Description: |
JP 2002536024-A/17: Test kit and method for quantitatively detecting genetically modi-fied DNA in foodstuff by means of fluorescence-coupled PCR. |
Taxon: |
synthetic construct |
Original size: |
250 bp |
Patents: |
JP2002536024 (lens, espacenet)
|
View other 7 matches for this record
Method: |
QT-TAX-GM-002 |
Go to GMOMETHODS record |
Description: | Quantitative PCR method for detection of soybean lectin gene |
Target: | soybean lectin |
View other 20 matches for this method
Predicted amplification details: |
Mismatches |
Gaps |
From |
To |
Strand |
Amplicon size |
0 |
0 |
117 |
190 |
+ |
74 bp |
|
1 ccagcttcgc cgcttccttc aacttcacct tctatgcccc tgacacaaaa
51 aggcttgcag atgggcttgc cttc
View other 25 matches with an identical amplicon
Primers and probe alignment: |
Primer/Probe |
% Identity |
Length |
Mismatches |
Gaps |
From |
To |
Score |
primerA |
100.0 |
20 bp |
0 |
0 |
1 |
20 |
3.63e-09 |
primerB |
100.0 |
23 bp |
0 |
0 |
74 |
52 |
9.36e-11 |
probe |
100.0 |
24 bp |
0 |
0 |
23 |
46 |
2.75e-11 |
|
Related publications:
Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101.
Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.