Match details
| Record ID: |
BD170160.1 |
| Database: |
emblrelpat |
Go to original record |
| Description: |
WO 2002034943-A/73: Method of quantifying gene modification and standard molecule to be used therein. |
| Taxon: |
synthetic construct |
| Original size: |
1102 bp |
| Patents: |
WO2002034943 (lens, espacenet)
|
View other 8 matches for this record
| Method: |
QT-CON-00-007 |
Go to GMOMETHODS record |
|
View in JRC GMO-Matrix
|
|
| Description: | Quantitative PCR method for detection of the junction between a synthetic cry1A(b) gene and the Phospho-enol-pyruvate Carboxylase intron N.9. |
| Target: | cryIAb-PEPC |
View other 15 matches for this method
| Predicted amplification details: |
| Mismatches |
Gaps |
From |
To |
Strand |
Amplicon size |
| 0 |
0 |
852 |
951 |
- |
100 bp |
|
1 tgttcaccag cagcaaccag atcggcctga agaccgacgt gaccgactac
51 cacatcgatc aggtgtagga gctctagatc tgttctgcac aaagtggagt
View other 7 matches with an identical amplicon
| Primers and probe alignment: |
| Primer/Probe |
% Identity |
Length |
Mismatches |
Gaps |
From |
To |
Score |
| primerA |
100.0 |
20 bp |
0 |
0 |
1 |
20 |
5.00e-09 |
| primerB |
100.0 |
25 bp |
0 |
0 |
100 |
76 |
1.11e-11 |
| probe |
100.0 |
25 bp |
0 |
0 |
34 |
58 |
1.11e-11 |
|
Related publications:
Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101.
Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.
