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Food, Farming, Fisheries
European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: HJ722712.1
Database: emblrelpat Go to original record
Description: Sequence 6 from patent US 8653326.
Taxon: unidentified
Original size: 5345 bp
Patents: US8653326 (lens, espacenet)

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Method: QL-CON-00-014 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of the junction between the nos promoter and the neomycin phosphotransferase II gene
Target:P-nos-nptII

View other 1572 matches for this method

Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 2027 2178 + 152 bp
Amplicon: 152 bp 
1     ttcccctcgg tatccaatta gagtctcata ttcactctca atcaaagatc
51    cggcccatga tcatgtggat tgaacaagat ggattgcacg caggttctcc
101   ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa
151   tc

View other 32 matches with an identical amplicon

Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 23 bp 0 0 1 23 1.87e-10
primerB 100.0 22 bp 0 0 152 131 6.33e-10
probe 100.0 25 bp 0 0 129 105 1.62e-11

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.