Match details
| Record ID: | HJ692005.1 | |
|---|---|---|
| Database: | emblrelpat | Go to original record |
| Description: | Sequence 6 from patent US 8575434. | |
| Taxon: | unidentified | |
| Original size: | 16752 bp | |
| Patents: | US8575434 (lens, espacenet) | |
View other 13 matches for this record
| Method: | QT-EVE-ZM-026 | Go to GMOMETHODS record |
|---|---|---|
| View in JRC GMO-Matrix | ||
| Description: | Quantitative PCR method for detection of maize event 4114 (EURL GMFF, 2018). | |
| Target: | DP-004114-3 | |
View other 8 matches for this method
Predicted amplification details:
| Mismatches | Gaps | From | To | Strand | Amplicon size |
|---|---|---|---|---|---|
| 0 | 0 | 2359 | 2438 | + | 80 bp |
| Amplicon: | 80 bp |
|---|
1 gctttggagc ctctcgtttg tagcacttgc acgtagttac ccggaccgaa 51 gcttcaacac agatctgata gtttaaacgc
View other 8 matches with an identical amplicon
Primers and probe alignment:
| Primer/Probe | % Identity | Length | Mismatches | Gaps | From | To | Score |
|---|---|---|---|---|---|---|---|
| primerA | 100.0 | 22 bp | 0 | 0 | 1 | 22 | 3.46e-10 |
| primerB | 100.0 | 28 bp | 0 | 0 | 80 | 53 | 2.16e-13 |
| probe | 100.0 | 27 bp | 0 | 0 | 24 | 50 | 7.43e-13 |
Related publications:
- Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101.
- Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.