European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record CS079678.1 details
Record ID: CS079678.1
Database: emblrelpat Go to original record
Description: Sequence 2 from Patent EP1528105.
Taxon: Arabidopsis thaliana
Original size: 2709 bp
Patents: EP1528105 (lens, espacenet)

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Method QL-ELE-00-009 details
Method: QL-ELE-00-009 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description: Qualitative PCR method for detection of nopaline synthase terminator (Lipp et al.,2001).
Target: T-nos

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Predicted amplification details:

Predicted amplification details
Mismatches Gaps From To Strand Amplicon size
0 0 2567 2684 + 118 bp

Amplicon size
Amplicon: 118 bp
1     GCATGACGTT ATTTATGAGA TGGGTTTTTA TGATTAGAGT CCCGCAATTA
51    TACATTTAAT ACGCGATAGA AAACAAAATA TAGCGCGCAA ACTAGGATAA
101   ATTATCGCGC GCGGTGTC 

View other 13841 matches with an identical amplicon


Primers alignment:

Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 24 bp 0 0 2567 2590 1.56e-10
primerB 100.0 24 bp 0 0 2684 2661 1.56e-10


Related publications:

  • Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101.
  • Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.