Match details

Record ID: GZ668709.1
Database: emblrelpat Go to original record
Description: Sequence 39315 from patent US 8344211.
Taxon: unidentified
Original size: 701 bp
Patents: US8344211 (lens, espacenet)

Method: QT-TAX-ZM-004 Go to GMOMETHODS record
Description:Quantitative PCR method for detection of maize aldolase 1 gene.
Target:maize aldolase 1

View other 211 matches for this method


Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 405 473 - 69 bp

Amplicon: 69 bp
1     agggaggacg cctccctcct tgaggacatc aacaaaaggc tgcccatcct
51    tggtcttctg gtacagggt 

Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 17 bp 0 0 1 17 1.38e-07
primerB 100.0 22 bp 0 0 69 48 2.95e-10
probe 96.0 25 bp 0 1 21 44 1.31e-09


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.