Match details

Record ID: GZ655672.1
Database: emblrelpat Go to original record
Description: Sequence 17948 from patent US 8344211.
Taxon: unidentified
Original size: 709 bp
Patents: US8344211 (lens, espacenet)

Method: QT-TAX-ZM-004 Go to GMOMETHODS record
Description:Quantitative PCR method for detection of maize aldolase 1 gene.
Target:maize aldolase 1

View other 211 matches for this method


Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 433 503 - 71 bp

Amplicon: 71 bp
1     agggaggacg cctccctccc ctgaggacat caacaaaaag gcttgccatc
51    cttggtcttc tggtacaggg t 

Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 17 bp 0 0 1 17 1.42e-07
primerB 100.0 22 bp 0 0 71 50 3.04e-10
probe 96.3 27 bp 0 1 22 48 1.04e-10


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.