Match details

Record ID: GZ585327.1
Database: emblrelpat Go to original record
Description: Sequence 7251 from patent US 8299321.
Taxon: unidentified
Original size: 410 bp
Patents: US8299321 (lens, espacenet)

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Method: QT-TAX-GM-007 Go to GMOMETHODS record
Description:Quantitative PCR method for detection of soybean lectin gene
Target:soybean lectin

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
2 0 255 373 + 119 bp

Amplicon: 119 bp
1     gccctctact ccacccccat ccacatttgg gacaaagaaa ccggtagcgt
51    tgccagcttc gccgcttcct tcaacttcan cttctaagcc cctgacacaa
101   aaanggcttg caaatgggc 

View other 1 match with an identical amplicon


Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 19 bp 0 0 1 19 2.01e-08
primerB 90.5 21 bp 1 1 119 99 1.30e-05
probe 100.0 25 bp 0 0 55 79 1.32e-11


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.