Match details

Record ID:	GZ585327.1
Database:	emblrelpat	Go to original record
Description:	Sequence 7251 from patent US 8299321.
Taxon:	unidentified
Original size:	410 bp
Patents:	US8299321 (lens, espacenet)

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Method:	QT-TAX-GM-002	Go to GMOMETHODS record
Description:	Quantitative PCR method for detection of soybean lectin gene
Target:	soybean lectin

View other 20 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
1	0	307	381	+	75 bp

Amplicon:

75 bp

1     ccagcttcgc cgcttccttc aacttcanct tctaagcccc tgacacaaaa
51    anggcttgca aatgggcttg ccttc

View other 3 matches with an identical amplicon

Primers and probe alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	3.68e-09
primerB	95.7	23 bp	1	0	75	53	4.42e-09
probe	91.7	24 bp	2	0	23	46	1.68e-08

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101.

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.