Match details
Record ID: |
GY311592.1 |
Database: |
emblrelpat |
Go to original record |
Description: |
Sequence 25 from patent US 7989607. |
Taxon: |
unidentified |
Original size: |
6186 bp |
Patents: |
US7989607 (lens, espacenet)
|
View other 12 matches for this record
Method: |
QT-CON-00-005 |
Go to GMOMETHODS record |
|
View in JRC GMO-Matrix
|
Description: | Quantitative PCR method for detection of the junction between the phosphinothricin N-acetyltransferase gene and the CaMV 35S terminator. |
Target: | pat-CaMV T-35S |
View other 222 matches for this method
Predicted amplification details: |
Mismatches |
Gaps |
From |
To |
Strand |
Amplicon size |
0 |
0 |
5792 |
5939 |
+ |
148 bp |
|
1 gccagttagg ccagttaccc agatctgagt cgacctgcag gcatgccgct
51 gaaatcacca gtctctctct acaaatctat ctctctctat aataatgtgt
101 gagtagttcc cagataaggg aattagggtt cttatagggt ttcgctca
View other 31 matches with an identical amplicon
Primers and probe alignment: |
Primer/Probe |
% Identity |
Length |
Mismatches |
Gaps |
From |
To |
Score |
primerA |
100.0 |
21 bp |
0 |
0 |
1 |
21 |
2.08e-09 |
primerB |
100.0 |
24 bp |
0 |
0 |
148 |
125 |
5.36e-11 |
probe |
95.5 |
22 bp |
0 |
1 |
36 |
56 |
1.03e-07 |
|
Related publications:
Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101.
Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.