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Match details


QL-ELE-00-014 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of phosphinothricin N-acetyltransferase gene (Grohmann et al., 2009). Targets:bar<--->bar
View other 1975 matches for this method


Database:nt Go to original record
Description:Gossypium hirsutum transgenic clone LLcotton25 phosphinothricin N-acetyltransferase gene, complete cds.
Taxon:Gossypium hirsutum
Original size:4644 bp
View other 15 matches for this record

Predicted amplification details:

Mismatches Gaps From To Strand Amplicon size
0 0 3227 3286 + 60


1     acaagcacgg tcaacttccg taccgagccg caggaaccgc aggagtggac
51    ggacgacctc  
View other 3739 matches with an identical amplicon

Primers and probe alignment:

Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.000 19 0 0 1 19 1.06e-08
primerB 100.000 18 0 0 60 43 3.56e-08
probe 100.000 18 0 0 21 38 3.56e-08

For more information, see:
"JRC GMO-Amplicons: a collection of nucleic acid sequences related to genetically modified organisms." Database (Oxford). 2015 Sep 30;2015. link

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