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Food, Farming, Fisheries
European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: AY123624.1
Database: nt Go to original record
Description: Zea mays transgenic Pat (Pat) gene, partial cds.
Taxon: Zea mays
Original size: 747 bp

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Method: QT-EVE-ZM-006 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Quantitative PCR method for detection of maize event Bt11 (Mazzara et al., 2005).
Target:Bt11

View other 1 match for this method

Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 382 451 + 70 bp
Amplicon: 70 bp 
1     gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
51    ctcatggagg gattcttgga

View other 1 match with an identical amplicon

Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 20 bp 0 0 1 20 3.42e-09
primerB 100.0 20 bp 0 0 70 51 3.42e-09
probe 100.0 27 bp 0 0 28 54 6.44e-13

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.