JRC GMO-Amplicons | EURL GMFF

Record ID:	AJ346927.1
Database:	nt	Go to original record
Description:	Hallea stipulosa chloroplast DNA for trnT-L-F region, specimen voucher Razafimandimbison 295 (LBR, MO, P, TAN)
Taxon:	Fleroya stipulosa
Original size:	1731 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
1	0	747	1318	+	572 bp

Amplicon:

572 bp

1     cgaaattggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acccactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aaagggcaat cctgagccaa atcctgtttt ccgaaaccaa aggttcagaa
151   agtgaaaaag ggataggtgc agagactcaa cggaagctgt tctaacaaat
201   ggagttagat gcattagtag agagagatct ttccatcgaa aaatcagaaa
251   gaatgaagtg aaggataaac gtatatacat acgtattgaa tactatatca
301   aatgattaat gacgacccga ctgaatctgt atttttatat atgaaaaacg
351   gaagaattgg tgtgaataga ttccacattg aagaaagatc gaatattcat
401   tgatcaaatg attcactcca tagtctgata gatcttttca agaactgatt
451   aatcggacga gaataaagat agagtcccat tctacatgtc aatgccggca
501   acaatgaaat ttatagtaag aggaaaatcc gtcgacttta aaaatcgtga
551   gggttcaagt ccctctatcc cc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	95.0	20 bp	1	0	1	20	1.20e-06
primerB	100.0	20 bp	0	0	572	553	2.58e-08

Match details