JRC GMO-Amplicons | EURL GMFF

Record ID:	AJ414549.1
Database:	nt	Go to original record
Description:	Cephalanthus natalensis chloroplast trnT-L-F region
Taxon:	Cephalanthus natalensis
Original size:	1730 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
1	0	747	1316	+	570 bp

Amplicon:

570 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acccactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aaagggcaat cctgagccaa atcctgtttt ccgaaaccaa aggttcagaa
151   agtgaaaaag ggataggtgc agagactcaa cggaagctgt tctaacaaat
201   ggagttagat gcattagtag aaagatcttt ccatcgaaaa ttcagaaaga
251   atgaagtgaa ggataaacgt atatacatac gtattgaata ctatatcaaa
301   tgattaatga cgacccgact gaatctgtat tttatatatg aaaaacggaa
351   gaattggtgt gaatagattc cacattgaag aaagaatcga atattcattg
401   atcaaatgat tcactccata gtctgataga tcttttcaag aactgattaa
451   tcggacgaga ataaagatag agtcccattc tacatgtcaa tgccggcaac
501   aatgaaattt atagtaagag gaaaatccgt cgacttttaa aatcgtgagg
551   gttcaagtcc ctctattccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.57e-08
primerB	95.0	20 bp	1	0	570	551	1.19e-06

Match details