JRC GMO-Amplicons | EURL GMFF

Record ID:	AJ414548.1
Database:	nt	Go to original record
Description:	Adina pilulifera chloroplast trnT-L-F region
Taxon:	Adina pilulifera
Original size:	1731 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
1	0	744	1322	+	579 bp

Amplicon:

579 bp

1     cgaaattggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acccactaag tgataacttt caaattcaga gaaaccctgg aattawtaam
101   aaagggcaat cctgagccaa atcctgtttt ccgaaaccaa aaggcaaagg
151   ttcagaaagt gaaaaaaggg ataggtgcag agactcaacg gaagctgttc
201   taacaaatgg agttagatgc attagtagag agatctttcc atcgaaaatt
251   cagaaagaat gaagtgaagg ataaacgtat atacatacgt attgaatact
301   atatcaaatg attaatgacg acccgactga atctgtattt ttatatatga
351   aaaacggaag aattggtgtg aatagattcc acattgaaga aagaatcgaa
401   tattcattga tcaaatgatt cactccatag tctgatagat cttctcaagn
451   actgattaat cggacgagaa taaagataga gtcccattct acatgtcaat
501   cgccgcaaca atgaaattta tagtaagagg aaaatccgtc gactttaaaa
551   atcgtgaggg ttcaagtccc tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	95.0	20 bp	1	0	1	20	1.21e-06
primerB	100.0	20 bp	0	0	579	560	2.61e-08

Match details