JRC GMO-Amplicons | EURL GMFF

Record ID:	AJ430898.1
Database:	nt	Go to original record
Description:	Cardiopteris quinqueloba chloroplast tRNA-Leu gene for transfer RNA-Leu and partial tRNA-Phe gene for transfer RNA-Phe
Taxon:	Cardiopteris quinqueloba
Original size:	1282 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
1	0	317	888	+	572 bp

Amplicon:

572 bp

1     cgaaattggt agacgctacg gacttaattg aattgagcct tggtacggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   catgggcaat cctgagccaa atcctgtttt caaaaaacaa accaaggttc
151   aaaaagcaaa aataaaaaga aaggataggt gcagagactc aatggaagct
201   gttctaacaa atggagttga gtgtgttgtg ttagtagagg aatccttcta
251   tcgaaacttc agaaaggatg aagataaacg tatatacgta ttgaaatact
301   atatcaaatg attaatgaca acgcgaatct gtatttttct attaaaaagg
351   gaagaattcc tgtgaatcga ttccacattg aagaaagaat caaaattcat
401   tgatcaaatt attcactccg cagtctgata gatcttttga agaactgatt
451   aattagatga gaataaagat agagtcccgt tctacatgtc actaccggca
501   acaatgcaat ttatagtaag aggaaaatcc gtcgacctta gaaatcgtga
551   gggttcaagt ccctctatcc cc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	95.0	20 bp	1	0	1	20	1.20e-06
primerB	100.0	20 bp	0	0	572	553	2.58e-08

Match details