Match details
| Record ID: |
EU090197.1 |
| Database: |
nt |
Go to original record |
| Description: |
Brassica napus trangenic line Rf2 right border junction sequence of transgenic event genomic sequence. |
| Taxon: |
Brassica napus |
| Original size: |
2943 bp |
| Method: |
QT-EVE-BN-007 |
Go to GMOMETHODS record |
|
View in JRC GMO-Matrix
|
|
| Description: | Quantitative PCR method for detection of oilseed rape event Rf2 (verified by the EU-RL GMFF in the context of Commission Decision 2007/306/EC) |
| Target: | RF2 |
| Predicted amplification details: |
| Mismatches |
Gaps |
From |
To |
Strand |
Amplicon size |
| 0 |
0 |
213 |
316 |
- |
104 bp |
|
1 gggtgagaca atatatcgac gtatatatag ctgtgccagt acaatattac
51 tcaccggcca aattcgctct tagccgtaca atattactca ccggtgcgat
101 gccc
| Primers and probe alignment: |
| Primer/Probe |
% Identity |
Length |
Mismatches |
Gaps |
From |
To |
Score |
| primerA |
100.0 |
21 bp |
0 |
0 |
1 |
21 |
1.54e-09 |
| primerB |
100.0 |
18 bp |
0 |
0 |
104 |
87 |
5.84e-08 |
| probe |
100.0 |
26 bp |
0 |
0 |
52 |
77 |
3.37e-12 |
|
Related publications:
Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101.
Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.
