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Food, Farming, Fisheries
European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: NRN_CS492094
Database: nrnl1 Go to original record
Description: Sequence 1 from Patent WO2007017186. Sequence 1 from patent US 7932439. Sequence 1 from Patent EP2295587.
Taxon: unidentified
Original size: 1214 bp
Patents: WO2007017186 (lens, espacenet) US7932439 (lens, espacenet) EP2295587 (lens, espacenet)
Method: QT-EVE-GH-006 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Quantitative PCR method for detection of cotton event GHB614 (Savini et al., 2008).
Target:GHB614

View other 5 matches for this method

Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 705 824 + 120 bp
Amplicon: 120 bp 
1     caaatacact tggaacgact tcgttttagg ctccatggcg atcgctacgt
51    atctagaatt cctgcaggtc gagtcgcgac gtacgttcga acaattggtt
101   ttaaaagctt gcatgcctgc

View other 5 matches with an identical amplicon

Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 24 bp 0 0 1 24 4.30e-11
primerB 100.0 21 bp 0 0 120 100 1.79e-09
probe 96.7 30 bp 0 1 31 60 4.25e-12

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.