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Food, Farming, Fisheries
European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: AR275289.1
Database: emblrelpat Go to original record
Description: Sequence 18 from patent US 6506963.
Taxon: unidentified
Original size: 537 bp
Patents: US6506963 (lens, espacenet)
Method: QT-EVE-BN-002 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Quantitative PCR method for detection of oilseed rape event Ms8 (Mazzara et al., 2007).
Target:Ms8

View other 13 matches for this method

Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 79 208 + 130 bp
Amplicon: 130 bp 
1     gttagaaaaa gtaaacaatt aatatagccg gctatttgtg taaaaatccc
51    taatataatc gacggatccc cgggaattcc gggggaagct tagatccatg
101   gatttgttat gataaccaaa aacaccctcc

View other 13 matches with an identical amplicon

Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 31 bp 0 0 1 31 8.34e-15
primerB 100.0 20 bp 0 0 130 111 6.57e-09
probe 100.0 28 bp 0 0 52 79 3.42e-13

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.