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Food, Farming, Fisheries
European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: HL351136.1
Database: emblrelpat Go to original record
Description: Sequence 1 from patent US 9204599.
Taxon: unidentified
Original size: 8557 bp
Patents: US9204599 (lens, espacenet)
Method: QT-EVE-ZM-004 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Quantitative PCR method for detection of maize event DAS-40278-9 (Savini et al., 2012).
Target:DAS-40278-9

View other 18 matches for this method

Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 1820 1917 + 98 bp
Amplicon: 98 bp 
1     cacgaaccat tgagttacaa tcaacagcac cgtaccttga agcggaatac
51    aatgaaggtt agctacgatt tacagcaaag ccagaataca atgaacca

View other 18 matches with an identical amplicon

Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 22 bp 0 0 1 22 4.29e-10
primerB 100.0 23 bp 0 0 98 76 1.26e-10
primerB 100.0 12 bp 0 0 56 45 1.64e-04
probe 100.0 25 bp 0 0 67 43 1.08e-11
probe 100.0 12 bp 0 0 95 84 1.83e-04

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.