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Food, Farming, Fisheries
European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: AX685147.1
Database: emblrelpat Go to original record
Description: Sequence 8 from Patent WO0236831.
Taxon: synthetic construct
Original size: 474 bp
Patents: WO0236831 (lens, espacenet)

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Method: QT-EVE-BN-004 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Quantitative PCR method for detection of oilseed rape event GT73 (Mazzara et al., 2007).
Target:GT73

View other 5 matches for this method

Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 183 290 + 108 bp
Amplicon: 108 bp 
1     ccatattgac catcatactc attgctgatc catgtagatt tcccggacat
51    gaagatcatc ctccttcctt tccttgcctt tccttccttt tcttgccttc
101   gtataagc

View other 5 matches with an identical amplicon

Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 26 bp 0 0 1 26 3.30e-12
primerB 100.0 24 bp 0 0 108 85 4.10e-11
probe 100.0 27 bp 0 0 40 66 9.65e-13

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.